Effects of midazolam pretreatment on inflammatory responses and cell apoptosis during intestinal ischemia-reperfusion in mice
10.3760/cma.j.issn.0254-1416.2016.03.029
- VernacularTitle:咪达唑仑预先给药对小鼠肠缺血再灌注时炎性反应和细胞凋亡的影响
- Author:
He ZHANG
;
Yulan CHEN
;
Jie TANG
;
Peng LOU
;
Jingyu CHANG
;
Bei MIAO
- Publication Type:Journal Article
- Keywords:
Midazolam;
Reperfusion injury;
Intestine;
Inflammation;
Apoptosis
- From:
Chinese Journal of Anesthesiology
2016;36(3):362-364
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of midazolam pretreatment on inflammatory responses and cell apoptosis during intestinal ischemia-reperfusion (I/R) in mice.Methods Thirty healthy male Kunming mice,weighing 18-22 g,were equally and randomly divided into 3 groups using a random number table:sham operation group (S group),I/R group,and midazolam pretreatment group (M group).Intestinal I/R was produced by occlusion of the superior mesenteric artery for 20 min followed by reperfusion.In group M,midazolam 1 mg/kg was injected intraperitoneally,and intestinal I/R was produced 30 min later.At 24 h of reperfusion,the mice were sacrificed,and intestinal tissues were removed for microscopic examination and for determination of the expression of interleukin-6 (IL-6),tumor necrosis factor-alpha (TNF-α) and caspase-3.Intestinal damage was assessed and scored according to Chiu.Results Compared with group S,Chiu's scores were significantly increased,and the expression of IL-6,TNF-α and caspase-3 was significantly up-regulated in I/R and M groups (P＜0.05).Compared with group I/R,Chiu's scores were significantly decreased,and the expression of IL-6,TNF-α and caspase-3 was significantly down-regulated in group M (P＜0.05).Conclusion Midazolam pretreatment can reduce intestinal I/R injury,and the mechanism is related to inhibition of inflammatory responses and cell apoptosis in mice.
