Radiosensitizing effect and mechanism of 2'-hydroxyflavanone in prostate cancer cells
10.3760/cma.j.issn.1004-4221.2016.05.020
- VernacularTitle:2'-羟基黄烷酮对前列腺癌细胞的放射增敏作用及机制探讨
- Author:
Wen WANG
;
Wei XIONG
;
Xiaoying LI
;
Xianshu GAO
;
Shaoqian SUN
;
Yi LI
- Publication Type:Journal Article
- Keywords:
2'-hydroxyflavanone;
Radiosensibility;
Prostate cancer cells line
- From:
Chinese Journal of Radiation Oncology
2016;25(5):513-518
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the radiosensitizing effect of 2'-hydroxyflavanone (2'-HF) on prostate cancer cells,and to preliminarily investigate its mechanism.Methods Colony formation assay,tert-butylhydroperoxide (TBHP) oxidative stress assay,Hoechst staining,and apoptosis flow cytometry using Annexin V-FITC and propidium iodide (PI) were performed to measure the impact of 2'-HF on the radiosensitivity of VCaP prostate cancer cells.Western blot was used to determine the effects of 2'-HF on expression of AKT,phosphorylated AKT (p-AKT),and aldo-keto reductase 1 C3 (AKR1 C3) in VCaP cells and preliminarily investigate the mechanism.Data were analyzed by t test and factorial analysis of variance.Results The results of colony formation assay indicated that after exposure to radiation,VCaP cells treated with 2'-HF had a significantly lower proliferation level than cells in the control group (P=0.010),yielding a sensitization enhancement ratio of 1.19.The resuhs of TBHP oxidative stress assay suggested that VCaP cells treated with 2'-HF had significantly weaker anti-oxidative capacity than cells in the control group (P=0.015).Hoechst staining and apoptosis flow cytometry with Annexin V-FITC and PI indicated that 2'-HF treatment plus irradiation significantly enhanced apoptosis in VCaP cells (P=0.001.The results of Western blot suggested that 2'-HF treatment significantly inhibited the protein expression of p-AKT and AKR1C3 in VCaP cells (P=0.013 and P=0.016).Conclusions 2'-HF can enhance the radiosensitivity of prostate cancer cells,which is probably associated with its inhibitory effects on AKT pathway and AKR1C3 expression in prostate cancer cells.
