Simultaneous Determination of Five Components in Qiangshenpaidu Capsules by UPLC-MS/MS
10.3870/j.issn.1004-0781.2016.07.024
- VernacularTitle:超高效液相色谱-串联质谱法同时测定强肾排毒胶囊5种成分含量?
- Author:
Zhitao JIANG
;
Guoliang DAI
;
Jinhuo PAN
;
Jianchun WANG
;
Xiaofeng CHEN
;
Hui YU
;
Wenzheng JU
- Publication Type:Journal Article
- Keywords:
Qiangshen paidu capsules;
UPLC-MS/MS;
Adenosine;
Cytidine;
Guanosine;
Mannitol;
Adenine
- From:
Herald of Medicine
2016;35(7):772-775
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an UPLC-MS/MS method for simultaneously determination of five components (adenosine,cytidine,guanosine,mannitol and adenine) in Qiangshenpaidu capsules. Methods The UPLC separation was performed on an Agilent ZOBAX SB-C18(2.1 mm×150 mm,5 μm) column.Isocratic elution was carried out with mobile phase consisting of methanol-0.1%formic acid (5∶95) at a flow rate of 0.2 mL.min-1.The mass spectrometer was operated in the positive ionization electrospray ( ESI) mode using multiple monitoring ( MRM) for analysis of five components. Results Adenosine,cytidine,guanosine,mannitol and adenine were all analyzed with good precision and accuracy. The linear ranges were 35-1 120 ng.mL-1( r=0.998 1) ,10-320 ng.mL-1( r=0.996 4) , 30-980 ng.mL-1( r=0.999 3) , 40-1 280 ng.mL-1( r=0.993 4), 25-800 ng.mL-1(r=0.996 5),respectively.The recoveries of six analytes ranged from 97.4% to 103.6% and the relative standard deviations were all below 4.7%. Conclusion A sensitive,accurate and suitable UPLC-MS/MS method has been developed,and the method could be applied for the determination of adenosine,cytidine,guanosine,mannitol,and adenine in Qiangshenpaidu Capsules.
