The effect of TNF-alpha on oligodendrocyte progenitor and its mechanism
10.3969/j.issn.1671-8348.2016.16.005
- VernacularTitle:肿瘤坏死因子-α对少突胶质前体细胞的影响及其机制的研究
- Author:
Feng WANG
- Publication Type:Journal Article
- Keywords:
tumor necrosis factor-alpha;
oligodendrocyte progenitor;
tumor necrosis factor receptor type 1;
periventricular leukomalacia
- From:
Chongqing Medicine
2016;45(16):2173-2175
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of tumor necrosis factor‐α(TNF‐α) on the oligodendrocyte progenitor ,and pro‐vide a strategy for the treatment of Periventricular leukomalacia .Methods The purified oligodendrocyte progenitor were divided in‐to three groups :blank control group ,TNF‐αgroup and anti‐TNFR1 group .TNF‐αgroup and anti‐TNFR1 group were treated with TNF‐α,the differentiation of OPC were detected by immunocytochemical method ,the relative viability of cells in three groups were measured by MTT assay ,the levels of TNFR1 gene was observed with RT‐PCR .Results The relative cell viability of TNF‐αgroup was significantly decreased(P<0 .05) and did not turn into cells called pro‐oligodendrocyte .TNF‐αgroup increased the expression of TNFR1 mRNA ,and there were no differences in the expression of TNFR1 mRNA between the blank control group and anti‐TNFR1 group .Conclusion TNF‐αcould induced OPC apoptosis and inhibited the differentiation of OPC through the TNFR1 .
