Protective effect of propofol preconditioning on limb ischemia reperfusion injury in rats

VernacularTitle:丙泊酚预处理对大鼠肢体缺血再灌注损伤的影响
Author: Zhe LI ; Yijun LU ; Liwen LYU ; Guohao LU ; Wei LI ; Ning YU ; Junyu LU
Publication Type:Journal Article
Keywords: propofol; limb; ischemia-reperfusion injury; inflammatory factor; oxygen free radicals
From: Chongqing Medicine 2016;45(17):2337-2339
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of propofol on rat′s limb ischemia reperfusion injury .Methods Sixty healthy SD rats were randomly divided into sham operate group ,ischemia-reperfusion group and propofol group (n= 20) ,each group was divided into 4 subgroups according to the different reperfusion time .To copy the right lower limb ischemia reperfusion model ,5 min before reperfusion ,use propofol injection (50 mg/kg ,intraperitoneal inject) ,various subjects in the corresponding time points (3 ,6 , 9 ,12 h) were sacrificed .TNF-α ,NF-κB of blood and MDA ,SOD of Skeletal muscle were measured ,calculate muscle wet dry weight ratio .Results Compared with ischemia reperfusion group ,propofol could significantly reduce expression of TNF-alpha ,NF-κB lev-els in serum (P< 0 .05) ,inhibit the increase of the MDA level and decrease of the SOD level in muscle (P< 0 .05) ,also reduce the extent of skeletal muscle cell edema(P< 0 .05) .Conclusion Propofol can attenuate limb ischemia reperfusion injury by inhibiting inflammation response and reducing the oxygen free radicals′ damage .