The role of polymerase chain reaction with confronting two-pair primers in SNP genotyping of DNA base excision repair genes
10.3969/j.issn.1671-8348.2016.16.021
- VernacularTitle:PCR-CTPP在DNA 碱基切除修复基因SNP分型中的应用
- Author:
Yang PENG
;
Yi CHENG
;
Yuxin YANG
;
Chongyi LI
;
Mengxia LI
;
Shiheng ZHANG
;
Dong WANG
- Publication Type:Journal Article
- Keywords:
polymorphism,single nucleotide;
polymerase chain reaction with confronting two-pair primers;
base excision repair
- From:
Chongqing Medicine
2016;45(16):2226-2228
- CountryChina
- Language:Chinese
-
Abstract:
Objective It is important to precisely determinate the single nucleotide polymorphisms (SNPs) in many genes in‐cluding genes related with base excision repair (BER) pathway .This research is conducted to evaluate the role of polymerase chain reaction with confronting two‐pair primers (PCR‐CTPP) in analyzing the SNPs of BER pathway .Methods Four common SNPs of BER pathway (OGG1 Ser326Cys ,XRCC1 Arg399Gln ,APE1 Asp148Glu and‐141T/G in the promoter region) was detected with PCR‐CTPP .10 of the products were sent for genotype sequencing .Compare the results of PCR and sequencing to evaluate the accu‐racy of PCR‐CTPP .Results The genotypes were exactly the same as the sequencing .Conclusion The PCR‐CTPP was a reliable and rapid detective technology for SNPs genotyping .Its broadest application would be great help for gene variant analysis .
