Comparison of Ziehl-Neelsen Stain and TB-PCR on Detection of Mycobacterium tuberculosisin Formalin-fixed, Paraffin-embedded Tissues of Chronic Granulomatous Inflammation.
- Author:
Min Sun CHO
1
;
Shi Nae LEE
;
Sun Hee SUNG
;
Woon Sup HAN
Author Information
1. Department of Pathology, College of Medicine, Ewha Womans University, Seoul, Korea. woonshan@shinbiro.com
- Publication Type:Original Article
- Keywords:
Chronic Granulomatous Diseases;
Mycobacterium tuberculosis;
Stains;
PCR
- MeSH:
Coloring Agents;
Diagnosis;
Granuloma;
Granulomatous Disease, Chronic;
Inflammation*;
Mycobacterium tuberculosis;
Mycobacterium*;
Necrosis;
Polymerase Chain Reaction;
Sensitivity and Specificity;
Tuberculosis
- From:Korean Journal of Pathology
2003;37(6):379-383
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: TB-PCR is a faster and more sensitive method to detect mycobacterium than acid-fast bacilli (AFB) stain, which is laborious and time consuming. We compared the sensitivity and specificity of AFB stain and TB-PCR and examined the possibility of TB-PCR as a confirmative test without AFB stain in the diagnosis of tuberculosis. METHODS: We performed Ziehl-Neelsen stain and nested PCR using a commercially available TB-PCR kit amplifying IS6110 sequence in 81 cases of paraffin-embedded tissues diagnosed as chronic granulomatous inflammation. In addition, we evaluated the morphology of granuloma and the presence of caseation necrosis. RESULTS: Of the 81 cases studied, 22 (27.2%) and 40 (49.4%) were positive for AFB stain and TB-PCR, respectively. Of 49 cases accompanying caseation necrosis, 19 (38.8%) were AFB stain positive and 37 (75.5%) were TB-PCR positive; a result that is comparable with that of other reports. Of the 22 AFB-positive cases, 2 were TB-PCR negative. CONCLUSION: TB-PCR is very helpful for the diagnosis of tuberculosis in routinely processed, formalin-fixed, paraffin-embedded tissue samples. Nevertheless, AFB stain should continue to be performed at the same time.
