Protective effects of choline on myocardial ischemic rat heart and its potential mechanisms

VernacularTitle:M 3受体激动剂对急性缺血性心肌的保护作用
Author: Hairong LUAN ; Jian SUN ; Deli WANG ; Li LI ; Hong WU
Publication Type:Journal Article
Keywords: myocardial ischemia; choline; ICa-L; patch clamp technique; laser scanning confocal microscope
From: Chongqing Medicine 2016;45(17):2305-2307
CountryChina
Language:Chinese
Abstract: Objective To study the protective effects of choline on myocardial ischemia rat heart and its potential mecha -nisms .Methods Ischemia hypoxia environment was simulated with low value of pH (pH 6 .8) and lack of oxygen .Calcium currents were recorded by whole cell patch under the voltage clamp configuration .The alternations in[Ca2 + ]induced by KCl was detected by laser scanning confocal microscope in ventricular myocytes ,then disccuss the effects of choline on calcium and calcium store in cells . Results The normalized peak currents of ICa-L in ventricular myocytes were larger in pH 6 .8 group than those in pH 7 .4 group , which can be attenuated by choline .The(Ca2 + )i induced by KCl in ventricular myocytes were significantly increased in pH 6 .8 Ty-rode solution and its increasing can be suppressed by choline .4-DAMP can inhibit the suppressing effect of choline .Conclusion The possible mechanism of M 3 receptor involved in the protection of ischemic myocardium by inhibiting myocardial cells in ICa-L ,in-hibiting intracellular calcium overload .