The study on association between epithelial mesenchymal transition and Oxaliplatin resistance for colorectal carcinoma
10.3969/j.issn.1671-8348.2016.17.002
- VernacularTitle:上皮间质转化与大肠癌奥沙利铂化疗耐药的关系研究
- Author:
Houjun JIA
;
Lin XIANG
- Publication Type:Journal Article
- Keywords:
epithelial mesenchymal transition;
colorectal carcinoma;
Oxaliplatin
- From:
Chongqing Medicine
2016;45(17):2308-2311
- CountryChina
- Language:Chinese
-
Abstract:
Objective Drug resistance is the main reason for chemotherapy failure ,it is to be solved how to overcome this . Recent studies indicate that epithelial-mesenchymaltransition (EM T ) may involved in chemotherapy resistance for some types of cancers .But it is still unknown whether EM T is involved in chemotherapy resistance for colorectal carcinoma .This study was con-ducted to investigate the relation between EM T and colorectal cancer chemotherapy resistance .Methods Abilities of migration and invasion of Oxaliplatin resistant colorectal carcinoma cell lines LOVO /L-OHP and wild type colorectal carcinoma cell lines LOVO were investigated by transwell migration and transwell invasion assays .The level of E-cadherin and Vimentin was detected by West-ern blot .EM T of LOVO was induced by GSK-3β inhibitor SB415286 treatment and validated by transwell migration and transwell invasion assays and Western blot for E-cadherin and Vimentin .The sensitivity of LOVO to Oxaliplatin after SB 415286 inducing EM T was evaluated by M TT and Annexin-V /PI assays .Results EM T phenotype was confirmed by morphology feature ,results of transwell migration and invasion assays and level changes of E-cadherin and Vimentin .In addition ,the results of proliferation and apoptosis tests showed that the sensitivity of LOVO to Oxaliplatin after SB 415286 inducing EM T decreased significantly ,which means chemotherapy resistance .Conclusion The direct association between EM T and chemotherapy resistance for colorectal carci -noma is proved in this study ,which provides basis for intervening chemotherapy resistance and new researching strategies .
