Effection of HDAC1 deacetylase inhibition on gastric cancer stem cells

VernacularTitle:抑制组蛋白去乙酰酶1后对人胃癌干细胞的影响
Author: Xilu HOU ; Jun TANG ; Bin ZHU ; Hezhong YAN ; Senyuan YU ; Yan HE ; Haiqing LI ; Jiaoxue WANG ; Wei LIU
Publication Type:Journal Article
Keywords: HDAC1; gastric cancer stem cells; proliferation; differentiation; invasion
From: Chongqing Medicine 2016;45(17):2319-2322
CountryChina
Language:Chinese
Abstract: Objective To explore the effect of HDAC1 deacetylase inhibition on the proliferation differentiation and invasion in human gastric cancer stem cells (GCSCs) .Methods The GCSCs were selected as CD44 marker by using flow cytometry .RT-qPCR and Western Blot were used to detect the expression of HDAC 1 in GCSCs and non GCSCs .The effect of proliferation and in-vasion in GCSCs were observed by CCK-8 assay ,colony formation and transwell assay after the cells were treated with TSA .The expression of proteins related apoptosis ,differentiation and invasion were detected by using RT-qPCR and Western blot .Results The expression of HDAC1 in GCSCs was higher than that in non GCSCs .The capacities of proliferation and invasion in experimen-tal group were attenuated compared to the control group .The proteins related differentiation was down regulated ,and epithelial mesenchymal transition was mediated .Conclusion After the deacetylation of HDAC1 was inhibited ,the proliferation ,differentia-tion and invasion of GCSCs were reduced .