Effects of siRNA inhibit HMGA1 gene expression on LX-2 cell biological functions
10.3969/j.issn.1671-8348.2016.17.006
- VernacularTitle:siRNA 抑制 HM GA1基因表达对 LX-2细胞生物学功能的影响
- Author:
Lei HU
;
Li LIU
;
Shuang ZHANG
;
Fangteng DU
;
Jixiang ZHANG
- Publication Type:Journal Article
- Keywords:
high mobility group AT-hook 1;
RNAi;
hepatic stellate cell LX-2;
liver fibrosis
- From:
Chongqing Medicine
2016;45(17):2323-2326
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of siRNA mediated HMGA 1 silence on proliferation and the gene expression of HMGA1 ,α-SMA and E-cadherin in activated hepatic stellate cells and its mechanisms .Methods Synthetic HMGA1 siRNA was transfected into LX-2 cells to silence the HMGA1 gene .The expression level of HMGA1 ,α-SMA and E-cadherin was determined by RT-PCR and Western blot experiments .LX-2 cell proliferation was assessed by M TT assay .Results The best inhibited effect was HMGA1-siRNA-1 .Compared with control group ,the cell proliferation and the mRNA and protein expression of HMGA 1 ,α-SMA in TGF-β1 group and TGF-β1 + NC-siRNA group were significantly increased (P< 0 .05) ,without significant differences between the two groups (P> 0 .05) ,while the expression of E-cadherin in TGF-β1 group and TGF-β1 + NC-siRNA group were significantly decreased compared with control group (P< 0 .05) .Meanwhile ,the cells in TGF-β1 + HMGA1 siRNA group showed significantly decreased proliferation level ,down-regulated mRNA and protein expression of HMGA 1 ,α-SMA but up-regulated expression of E-cadherin compared with TGF-β1 group and TGF-β1 + NC-siRNA group(P< 0 .05) .Conclusion HMGA1 interference could signifi-cantly down-regulate the expression of HMGA1 in LX-2 cells cultured with TGF-β1 ,thus inhibiting the proliferation and activation of the cells .
