Expression and significance of neutrophil surface adhesion molecules CD11b in type 2 diabetes mellitus patients with cytomegalovirus infection
10.3760/cma.j.issn.1008-6706.2016.15.007
- VernacularTitle:2型糖尿病合并巨细胞病毒感染患者中性粒细胞表面黏附分子 CD11b表达及临床意义
- Author:
Jun ZHANG
;
Lixin CHEN
;
Xiaoyun JI
;
Xiangping WU
;
Fei ZHENG
- Publication Type:Journal Article
- Keywords:
Type 2 diabetes mellitus;
Cytomegalovirus;
CD11b;
Flow cytometry
- From:
Chinese Journal of Primary Medicine and Pharmacy
2016;23(15):2264-2267
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study diagnosis significance of neutrophil surface adhesion molecules including CD11b,in type 2 diabetes mellitus patients with cytomegalovirus (CMV)infection.Methods Blood samples from 139 cases of type 2 diabetes mellitus patients were collected including 99 male and 40 female cases between Septem-ber 2011 and January 2015.Healthy male 20 cases and female 13 cases were collected as healthy control group from outpatients in the same period.Type 2 diabetes mellitus patients were divided into high active CMV infection group, CMV infection group and the negative control group,and healthy group as normal control group according to CMV -pp65 antigen detection.Neutrophil surface adhesion molecule CD11b was analyzed by flow cytometry and compared respectively.ROC curve of CD11b in 2 diabetes mellitus patients was made.Results The expression ratio of CD11b in high active CMV infection group and CMV infection group in type 2 diabetes mellitus patients was (41.25 ± 5.33)%.There was significant difference (P =0.019)compared with negative control group (61.13 ±5.28)% and there was significant difference (P =0.009)compared with negative control group (64.21 ±6.02)%;optimal cut -off value of CD11b in high active CMV infection group was 45.65%,the sensitivity was 100.00%,the specificity in high active CMV infection group was 60.12%,and the area under the receiver -operating curve(ROC)was 0.628;optimal cut -off value of CD11b in CMV infection group was 70.21%,the sensitivity of CD11b was 87.69%,the specificity of CD11b was 99.22%,and the area under of ROC of CD11b was 0.991.Conclusion The neutrophils CD11b expression in type 2 diabetes mellitus patients with CMV infection is down regulation.CD11b expression can be used as a laboratory diagnosis basis of CMV infection.
