Expressions of microRNA -126 in the blood plasma in patients with diabetic retinopathy and its mechanism
10.3760/cma.j.issn.1008-6706.2016.14.015
- VernacularTitle:微小 RNA-126在糖尿病视网膜病变患者血浆中的表达及作用机制研究
- Author:
Hui WANG
;
Yan WEN
;
Li YAN
;
Xiaoming YU
- Publication Type:Journal Article
- Keywords:
Diabetic retinopathy;
miR -126;
Plasma;
Vascular endothelial growth factor
- From:
Chinese Journal of Primary Medicine and Pharmacy
2016;23(14):2134-2137
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of microRNA -126(miR -126)in the blood plasma in patients with diabetic retinopathy (DR)and its possible mechanism.Methods 81 patients with DR,65 patients with type 2 diabetes and 50 healthy people (control group)were selected.The expression of miR -126 in plasma was detected by real -time PCR.The serum concentration of VEGF was detected by enzyme -linked immunosorbent assay (ELISA).The correlations between variables were analyzed by Pearson correlation analysis.The value of miR -126 in predicting the occurrence of DR was analyzed by using receiver operating characteristic curve (ROC curves).Results The expressions of miR -126 in plasma in DR patients and type 2 diabetes were lower than the control group,which in DR patients was lower than the type 2 diabetes,the serum concentrations of VEGF in DR patients and type 2 diabe-tes were higher than the control group,which in DR patients was higher than the type 2 diabetes,the differences were statistically significant (all P <0.05).Pearson correlation analysis showed that the expression of miR -126 in plasma was negatively correlated with the concentration of VEGF (r =-0.352,P <0.05).ROC curve analysis showed that the area under the curve for expression level of miR -126 in predicting DR occurrence was 0.848 (95% CI:0.776 -0.919),when the expression level of miR -126 ≤0.47,the sensitivity was 83.1%,and specificity was 80.2%. Conclusion The expression of miR -126 in plasma in DR was reduced,while the serum level of VEGF was elevated.The miR -126 might through negatively regulated VEGF to regulate retinal angiogenesis.It expected to be blood indicators for early detecting DR.
