Identification and renaming of one strain used for tracheitis vaccine production
10.3760/cma.j.issn.0254-5101.2016.06.009
- VernacularTitle:一株气管炎疫苗生产菌株的鉴定更名
- Author:
Jichun SHI
;
Huijing DU
;
Li LIANG
;
Kang LI
;
Xiao XU
;
Yuhua XING
;
Cuiping CHENG
;
Qiang YE
- Publication Type:Journal Article
- Keywords:
Tracheitis vaccine;
Bacteria identification;
Acinetobacter baumannii;
Branhamella catarrhalis
- From:
Chinese Journal of Microbiology and Immunology
2016;36(6):448-452
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify and rename one strain stored in National Center for Medicine Culture Collections ( CMCC) and used for tracheitis vaccine production. Methods The test strain CMCC (B)29108 and the type strain DSM30007T were cultured on NA medium. Characteristics in morphology, physiology, biochemistry and fatty acid profile were compared between the two strains. Phylogenetic analysis was based on 16S rRNA and rpoB gene sequences, together with the DNA-DNA hybridization assay. Results A Comparative analysis of a partial sequence of the 16S rRNA gene sequence revealed that the CMCC( B) 29108 strain was closed to the Acinetobacter species and showed the highest similarity with the type strain Acinetobacter baumannii DSM30007T. Moreover, the CMCC(B)29108 strain was highly similar to type strain DSM30007T in morphology, physiology, biochemistry and fatty acid profile. On the phylogenetic tree based on 16S rRNA and rpoB gene of all Acinetobacter members, the CMCC(B)29108 strain steadily clustered into one independent branch only with the DSM30007 T strain with a DNA-DNA hybridization value of 100%. Conclusion The CMCC(B)29108 strain that is one of the strains used for the production of tracheitis vac-cine should be assigned to the species of Acinetobacter baumannii based on its phenotypic, phylogenetic and chemotaxonomic characteristics.
