Role of NK-1 Receptor in Fos Protein Expression of Anterior Cingulate Cortex Neurons of Mice Induced by Amputation of the Tail Extremity
10.12007/j.issn.0258-4646.2016.08.007
- VernacularTitle:NK-1受体在截断尾末端小鼠扣带回前部神经元Fos蛋白表达中的作用
- Author:
Yajuan ZHANG
;
Minfan WU
;
Mengfei WU
;
Yu YANG
;
Lihong SHANG
;
Bing WANG
;
Jian PAN
- Publication Type:Journal Article
- Keywords:
anterior cingulate cortex;
phantom limb pain;
Fos protein;
immunohistochemistry;
NK-1 receptor
- From:
Journal of China Medical University
2016;45(8):700-703
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study whether amputation of the tail extremity could induce change of Fos protein expression in mice ACC neurons , and explore the role of NK?1 receptor in the change. Methods Immunohistochemistry technique was adopted to study Fos protein expression change in mice ACC neurons at 0.25 h,0.5 h,1 h,2 h after amputation of the tail extremity 2.5 cm,and also the effect of NK?1 receptor antagonist GR82334(iv)or GR82334(ith)in the change. Results Fos protein expression in mice ACC neurons was significantly increased at 0.25 h,0.5 h after the amputation,and reached its peak at 1 h after the amputation,then started to decrease at 2 h after the amputation. GR82334(iv)com?pletely antagonized the significant augment in Fos protein expression in mice ACC neurons after the amputation ,but the antagonism of GR82334 (ith)was incomplete. Conclusion Amputation of the tail extremity could significantly increase the Fos protein expression of mice ACC neurons in a time?dependent manner. Both peripheral and central NK?1 receptors were involved in the process. However ,there are also central conduction pathways of other receptors and neurotransmitters involved in the significant augment in Fos protein expression in mice ACC neurons after amputa?tion.
