CD34, acancer stem cell marker,in nasopharyngeal carcinoma celllines
10.3969/j.issn.2095-4344.2016.23.004
- VernacularTitle:恶性肿瘤干细胞标记物CD34在鼻咽癌细胞系的表达
- Author:
Junhong ZHANG
;
Qing LI
;
Chunhua WANG
;
Xiuyun WU
;
Xinge LU
;
Lifei WANG
;
Leilei YANG
;
Qiuhong WANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(23):3374-3379
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Previous research have confirmed that CD34 is closely related to oncogenesis, progress, recurrence, metastasis and drug-resistance of various cancers, but its role in nasopharyngeal carcinoma remains unclear.
OBJECTIVE:Tosortcels positive and negative for CD34 in nasopharyngealcarcinoma cel lines and to detect cel proliferation and migration.
METHODS:Expressionsof CD34 in nasopharyngeal carcinoma cel lines 5-8F, 6-10B, CNE1 and CNE2 were detected by flow cytometry. And CD34+and CD34-cels were sorted based on cel surfacemarkers for purity identification. Afterwards, proliferation and migrationof CD34+and CD34-celswere detected by MTT assay, colony-formation assay and scratch assay.
RESULTS AND CONCLUSION:Al four nasopharyngeal carcinoma cel lines expressed CD34 in
0.1%-0.2%, and the level of CD34 was closely related to the cel growth density. The purity of CD34+cel was more than 98% in the sorted CD34+celpopulations, but no CD34+cels were found inthe sorted
CD34-celpopulations.At 1, 3, 5 and 7 daystheproliferation rate of CD34+cel, populationswas
significantly higher than that of CD34-cels (P< 0.05). Consistently, thecolony-formation efficiencyof CD34+cel was significantlyhigher than that ofCD34-cels (P< 0.05). Moreover, CD34+cels migrated significantly faster than CD34-cels by scratch assay (P< 0.05). In conclusion, CD34+cels culturedin vitro display higher proliferation and migration capacities, indicating that CD34+celshavethe potential of nasopharyngeal carcinoma stem cels.
