Effect ofGuiqi Dihuang Tangon the treatment of sensorineural deafness with cochlear stem cells
10.3969/j.issn.2095-4344.2016.23.014
- VernacularTitle:归芪地黄汤干预对耳蜗干细胞治疗感音性耳聋的影响
- Author:
Hanqing ZHU
;
Junfeng ZHANG
;
Youdong CHEN
;
Zhen XU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(23):3439-3444
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Combining Chinese medicine with stem cel transplantation opens up a new avenue for the use oftraditional Chinese medicinein thestem cel transplantation. It is expected to improve survival and differentiationof cochlear stem celsintohair cels through Chinese medicine interventions.
OBJECTIVE:To observe the influenceofGuiqi Dihuang Tangon the treatment of sensorineural deafness with cochlear stem cels.
METHODS:Guinea pigs with sensorineural deafness were randomly assigned into three groups:combined group intervened with cochlear stem cel suspension with medicated serum, cel transplantation group with cochlear stem cel suspension, and blank control group with normal saline injection. At 7, 28 and 56 days after treatment, al guinea pigs were detected for auditory brainstem response and immunofluorescent observation.
RESULTS AND CONCLUSION:Nestin positive cels and MyosinVIIA positive cels were observed in the combined and cel transplantation groups, and the number of positive cels was higher in the combined group than the cel transplantation group. Auditory brainstem response threshold of guinea pigs was decreased in the combined and cel transplantation groups, and the recovery of hearing was better in the former group. These findings indicate that the intervention ofGuiqi Dihuang Tangcan remarkably improve the survival rate of transplanted stem cels and the differentiation ratioof transplanted celsintohair cels.
