The effect and mechanism of rt-PA combined with high pressure oxygen on cerebral ischemia-reperfusion inj ury in rats
10.7652/jdyxb201603015
- VernacularTitle:rt-PA联合高压氧对大鼠脑缺血再灌注损伤的保护作用及其机制
- Author:
Qingguo BI
;
Chunlan LIU
;
Jianhui FU
;
Qinghua LI
;
Xinjuan CHU
;
Weizhong XIAO
- Publication Type:Journal Article
- Keywords:
rt-PA;
high pressure oxygen;
combined therapy;
cerebral ischemia-reperfusion;
neurological protection
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2016;37(3):372-377
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect and mechanism of rt-PA combined with high pressure oxygen (HPO)on cerebral ischemia-reperfusion injury in rats.Methods The model of cerebral ischemia-reperfusion injury was constructed by using middle cerebral artery occlusion.The neurological function score;brain index,water content and infarction volume;SOD;LDH;NOS;MDA;LD;NO and NOS were measured.The protein and mRNA expressions of iNOS,BDNF,p75NTR and TrkB were also detected by RT-PCR and Western blot to evaluate and compare the protective effect of rt-PA combined HPO therapy. Results rt-PA combined HPO could significantly decrease the neurological function score;brain index,water content,and infarction volume;SOD;NOS;MDA;LD;NO and NOS but increase LDH content and the weight of rats,compared with rt-PA.In addition,rt-PA combined HPO could increase BNDF and TrKB expressions and downregulate the expressions of iNOS and p75NTR,compared with rt-PA (P<0.05).Conclusion The rt-PA combined HPO therapy has a greater protective effect than rt-PA therapy and its mechanism might be related to having antioxidant effects, increasing the expressions of BDNF and TrKB,and decreasing the expressions of iNOS and p75NTR.
