Establishment of Congenital Heart Disease Transposition of Great Arteries in Experimental Mice Model

VernacularTitle:先天性心脏病大动脉转位小鼠模型的建立
Author: Shijun GAO ; Yu NIE ; Hong LIAN ; Shengshou HU
Publication Type:Journal Article
Keywords: Heart delects,congenital; Transposition of great arteries; All-trans retinoic acid; Model,animals
From: Chinese Circulation Journal 2016;31(5):499-501
CountryChina
Language:Chinese
Abstract: Objective: To establish a mice model of congenital heart disease transposition of great arteries in order to provide a research reference for the occurrence and development of transposition of great arteries. Methods: A total of 20 pregnant ICR mice at 8-10 weeks of age were divided into 2 groups: Control group, the mice received a single dose of DMSO 70 mg/kg at 8.5 days of gestation,n=5 and Experiment group, the mice received a single dose of all-trans retinoic acid 70 mg/kg at 8.5 days of gestation,n=15. All animals were treated for 18 days and then the embryos were taken to observe cardiac morphology under stereomicroscope. Results: Compared with Control group, Experiment group had obviously increased occurrence rates of premature delivery, abortion and embryo absorption, and 61.2% phenotype for transposition of great arteries; meanwhile, combining with non-heart defect phenotypeas exophthalmos and spinal malformation. Conclusion: All-trans retinoic acid may induce transposition of great arteries in mice embryos, which is a feasible animal model in experimental research.