Effect of Combination of Epigallocatechin-3-gallate and Cisplatin on Proliferation and Apoptosis of SW620
10.3870/j.issn.1004-0781.2016.06.010
- VernacularTitle:表没食子儿茶素没食子酸酯联用顺铂对结肠腺癌细胞株SW620增殖和凋亡的影响
- Author:
Feng GAO
;
Xiumei SUN
- Publication Type:Journal Article
- Keywords:
Epigallocatechin-3-gallate;
Cisplatin;
Proliferation;
Apoptosis
- From:
Herald of Medicine
2016;35(6):596-600
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of different composed combination of EGCG and cisplatin on human colon adenocarcinoma cell line SW620 and the related mechanisms. Methods SW620 cells at exponential phase were treated with cisplatin(5 mmol.L-1)and EGCG(0, 25, 50 and 100 mmol.L-1).Seventy hours after the cultivation, cell proliferation was detected with MTT;the percentage of apoptotic cells was determined by flow cytometry;expression levels of JAK2, p-JAK2, p-STAT3, STAT3, Bax, Bcl-2, cleavage-PARP, and cleavage-caspase3 were detected by Western blotting. Results The SW620 cell proliferation rate was(95±5)%,(67±5)%,(42±5)%(22±4)% and the apoptotic rat was 5%, 15%, 35%, 57%in the 0, 25,50, 100 mmol.L-1 EGCG groups, respectively.Moreover, EGCG up-regulated the expression of Bax, cleavage-PARP and cleavage-caspase3(P<0.05)and down-regulated the expression of p-JAK2, p-STAT3 and Bcl-2(P<0.05)in a dose-dependent manner, but had no influence on the expression of JAK2 and STAT3(P>0.05). Conclusion EGCG can promote cisplatin-induced apoptosis and proliferation inhibition of human colon adenocarcinoma cell lines of SW620 by suppressing JAK2/STAT3 gignaling pathway.
