Simultaneous Ditermination of Three Indicators in Chrysanthemi Flos by Quantitative Analysis of Multi-Components by Single Marker
10.3870/j.issn.1004-0781.2016.06.022
- VernacularTitle:一测多评法测定菊花中3个指标成分含量
- Author:
Wei HUANG
;
Yi WANG
- Publication Type:Journal Article
- Keywords:
Chrysanthemum;
Quantitative analysis of multi-components by single marker;
Correction factor,relative;
Chlorogenic acid;
Chromatography,high performance liquid
- From:
Herald of Medicine
2016;35(6):645-649
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determination of chlorogenic acid, galuteolin and 3,5-O-dicaffeoyl quinic acid in chrysanthemums by quantitative analysis of multi-components by single marker ( QAMS ) . Methods Using chlorogenic acid as the internal standard substance, the relative correction factors ( RCFs ) of galuteolin and 3, 5-O-dicaffeoyl quinic acid were determined by HPLC.The contents of chlorogenic acid, galuteolin and 3,5-dicaffeoyl quinic acid in 12 samples of chrysanthemums were authentically determined by the external standard method, so as to to verify the accuracy of the QAMS method. Results The average relative error of contents of galuteolin and 3,5-O-dicaffeoyl quinic acid was 2.75% and 1.33%by the external standard method and QAMS, respectively. Conclusion The established RCFs have good reproducibility.In case of lack of standard substances, the QAMS method with chlorogenic acid as an internal standard for determination of 3 indicators in chrysanthemum is feasible.
