Relationship of plasma levels of microRNA 491-5p and its target gene polymorphism with the prognosis of patients with premature coronary artery disease
10.7652/jdyxb201603003
- VernacularTitle:microRNA-491-5p血浆水平及其靶基因多态性与早发冠心病易感性的研究
- Author:
Yan ZHANG
;
Congxia WANG
;
Weidong MA
;
Yongqin LI
;
Zhenhua HAN
;
Chunyan ZHANG
;
Haoyu WU
;
Xiaohui QUAN
;
Jing HUI
- Publication Type:Journal Article
- Keywords:
premature coronary artery disease;
micro RNAs;
single nucleotide polymorphism;
matrix metallo-proteinases-9(MMP-9);
miRNA-491-5p(has-miR-491-5p)
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2016;37(3):318-321
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship of the plasma levels of miRNA-491-5p in Han population in Shaanxi Province and the changes of single nucleotide polymorphisms (SNP ) of the target gene matrix metalloproteinases-9 (MMP-9 ) of miRNA-4 9 1-5 p (has-miR-4 9 1-5 p ) with the incidence risk and prognosis of premature coronary artery disease (pCAD)through the case-control design.Methods In this study,we made a consecutive recruitment of 270 pCAD cases in the case group and 300 cases in the control group.Using the polymorphism method of polymerase chain reaction and restriction fragment length (PCR-RFLP),target gene MMP-9 of has-miR-491-5p and rs1056628 genotypes was detected to compare the association between the variant genotypes and pCAD.Results In the changes of rs1056628C-A polymorphisms,compared with that of CC genotypes (the incidence was 42%),the risks of having coronary heart disease in the individuals carrying CA and AA genotypes were 31%,the difference was statistically significant (P=0.045).The risks of developing coronary heart disease in the individuals carrying CA and AA genotypes were reduced more significantly in the population with low total cholesterol (TC),and low low-density lipoprotein cholesterol (LDL-C).Conclusion Target gene MMP-9 of has-miRNA-491-5p rs1056628C-A polymorphism is associated with the reduced incidence risk of pCAD,and carrying C alleles is an independent risk factor for pCAD.
