Expression of CENP-W in gliomas and its effect on invasion of gliomas cells
10.7652/jdyxb201603018
- VernacularTitle:着丝粒蛋白W在脑胶质瘤中的表达及其对侵袭的作用
- Author:
Miaojing WU
;
Qiankun JI
;
Yanghua FAN
;
Shigang Lü
;
Minhua YE
;
Lei WU
;
Xingen ZHU
- Publication Type:Journal Article
- Keywords:
centromere protein W (CENP-W);
glioma;
invasion
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2016;37(3):388-392
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of CENP-W in gliomas and its relationship with clinicopathological parameters and prognosis and to explore the effects of centromere protein W (CENP-W)on the invasion of gliomas cells.Methods The expressions of CENP-W in high-grade glioma tissues,low-grade glioma tissues,and adjacent brain tissues were detected by real-time fluorescence quantitative PCR and Western blotting. The correlation of the expression of CENP-W with clinicopathological parameters and prognosis was analyzed statistically.Human gliomas U2 5 1 cells in vitro were transfected with small interfering RNA to downregulate the expression of CENP-W.The invasion and migration capabilities of gliomas cancer cells were assessed by Transwell assays.Results The expression level of CENP-W was significantly higher in glioma tissues than in normal tissues. There was a positive correlation between the three protein expression levels and the pathological grade of gliomas. CENP-W siRNA was successfully transfected into U2 5 1 cells.Compared with those of the cells transfected with the scramble siRNA and control cells,the invasive and migration activities were inhibited in the U2 5 1 cells transfected with CENP-W siRNA.The Kaplan-Meier analysis and Log-Rank test showed significant differences in progress free survival (PFS)between the CENP-W high-expression and low-expression groups.Conclusion The expression level of CENP-W was positively correlated with the pathological grade of gliomas and CENP-W can promote glioma cell invasion.It implicates that CENP-W can be a novel target in gliomas treatment.
