Curcumin inhibits AngⅡ-induced proliferation and oxidative stress in vascular smooth muscle cells
10.7652/jdyxb201603029
- VernacularTitle:姜黄素对血管紧张素Ⅱ诱导血管平滑肌细胞增殖及氧化应激的影响
- Author:
Chen WANG
;
Zhe MENG
;
Yanqiu MA
;
Ze LI
;
Hailong TAO
;
Zhongle BAI
;
Ling LI
- Publication Type:Journal Article
- Keywords:
curcumin (Cur);
vascular smooth muscle cells (VSMCs);
cell proliferation;
oxidative stress;
reac-tive oxygen species (ROS);
superoxide dismutase (SOD);
glutathione peroxidase (Gpx);
angiotensinⅡ(AngⅡ);
siRNA
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2016;37(3):441-446
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of curcumin (Cur)on AngⅡ-induced proliferation and oxidative stress of vascular smooth muscle cells (VSMCs).Methods Primary rat VSMCs were cultured and divided into control group,AngⅡ group,AngⅡ+Cur 5μmol/L group,AngⅡ+Cur 10μmol/L group,AngⅡ+Cur 20μmol/L group,and Cur 20μmol/L group.The proliferation of AngⅡ-induced VSMCs was measured by MTT assay.The mRNA and protein expressions of inducible nitric oxide synthase (iNOS)and p47phox were detected by real-time PCR and Western blot.Nitric oxide (NO)production was measured by Griess reaction.Production of intracellular reactive oxygen species (ROS)was measured by DCFH-DA staining,and the activities of superoxide dismutase (SOD)and glutathione peroxidase (Gpx)were detected by xanthine oxidase assay and visible spectrophotometer. small interfering RNA (siRNA)was used to silence the expression of p47phox to further explore the mechanism for Cur inhibiting the proliferation of AngⅡ-induced VSMCs and oxidative stress.Results VSMCs activities were not significantly affected by Cur at the concentration between 0 and 80μmol/L.Cur (5,10 and 20μmol/L)significantly inhibited AngⅡ-induced proliferation of VSMCs.Cur had an inhibitory effect on the overexpression of NO,iNOS, p47phox and ROS in VSMCs and upregulated the activities of SOD and Gpx in a concentration-dependent manner. AngⅡ-induced ROS production in VSMCs was significantly attenuated by pretreatment with p47phox specific siRNA.Conclusion Cur can inhibit the proliferation and oxidative stress of AngⅡ-induced VSMCs.
