Study on Composite Enzyme Assisted Extraction of Hyperoside from Acanthopanax sessiflorus
10.3870/j.issn.1004-0781.2016.06.020
- VernacularTitle:复合酶法辅助提取短梗五加叶中金丝桃苷?
- Author:
Enbo CAI
;
Jing XU
;
Xiaoman ZHENG
;
Yanan WANG
;
Dalong WANG
;
Manling ZHENG
;
Xiangxiang LIU
;
Yan ZHAO
- Publication Type:Journal Article
- Keywords:
Leaves of Acanthopanax sessiflorus;
Hyperoside;
Compound enzyme;
Orthogonal test
- From:
Herald of Medicine
2016;35(6):636-639
- CountryChina
- Language:Chinese
-
Abstract:
Objective To extract hyperoside from the leaves of Acanthopanax sessiliflorus by complex enzyme method, and optimize the extraction process by orthogonal experiment. Methods Hyperoside was determined by HPLC. Effects of temperature,α-amylase, neutral protease and cellulase on extraction rate were detected by the orthogonal tests, and the optimum extraction condition of hyperoside from the leaves of Acanthopanax sessiliflorus was determined by complex enzyme method. Results The main influence factor was temperature,follows byα-amylase, neutral protease and cellulase according to orthogonal analysis.The best condition was as follows: dose of cellulase, neutral protease and α-amylase was 2%, 0. 5% and 3%, respectively, extract at temperature of 30 ℃for 10 min. Under this condition, the extraction rate of hyperoside in the leaves of Acanthopanax senticosus was 0.52%. Conclusion As compared with the traditional technics, compound enzyme increases the productivity of hyperoside.
