Mechanism of Basic Fibroblast Growth Factor-chitosan Carrier Inducing Neural Stem Cells to Differentiate into Neurons
10.3969/j.issn.1006-9771.2016.05.009
- VernacularTitle:碱性成纤维细胞生长因子-壳聚糖载体诱导神经干细胞向神经元分化的机制
- Author:
Hongmei DUAN
;
Cong WANG
;
Zhaoyang YANG
;
Peng HAO
;
Junkui SHANG
;
Xiaoguang LI
- Publication Type:Journal Article
- Keywords:
basic fibroblast growth factor;
chitosan;
neuron;
neural stem cell;
differentiation;
rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2016;22(5):528-534
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the potential mechanism of basic fibroblast growth factor (bFGF)-chitosan carrier to induce neural stem cells to differentiate into neurons. Methods After purification, the neural stem cells were cocultured with chitosan, soluble bFGF and bFGF-chitosan carrier. Three hours, twenty-four hours, three days and seven days after induction, immunofluorescence staining of Nestin, beta tubulin III, microtubule-associated protein-2 (MAP2), and fibroblast growth factor receptor 1 (FGFR1) were used to observe the expres-sion of FGFR1;real-time fluorescent quantitative polymerase chain reaction (qRT-PCR) and Western blotting were used to detect RNA and protein level changes after induction. Results Three hours after induction, there was no significant difference in the expression of FGFR1 among three groups. Twenty-four hours after induction, the expression level of FGFR1 was significantly higher in the bFGF-chitosan carrier group than in the chitosan group and the soluble bFGF group (P<0.001);three days and seven days after induction, the expression of FGFR1 decreased significantly in the chitosan group and soluble bFGF group (P<0.001), however, it was still higher in the bFGF-chitosan carrier group;moreover, the expression of genes associated with the pathway of extracellular regulated protein kinases/mitogen activated protein ki-nase (Erk/MAPK) was significantly higher in the bFGF-chitosan carrier group than in the chitosan group and soluble bFGF group (P<0.001). Conclusion bFGF-chitosan carrier might upregulate the expression of FGFR1, then activate Erk/MAPK signal pathways, and finally promote the differentiation of neural stem cells into neurons.
