Protective effects of LiCl on synaptic atrophy induced by okadaic acid in differentiated SK-N-SH neuroblastoma cells
10.3969/j.issn.1001-1978.2016.05.015
- VernacularTitle:氯化锂对冈田酸所致SK-N-SH神经元突触萎缩的保护作用
- Author:
Yuting YUAN
;
Yingying SI
;
Zhanyang WANG
;
Xiangquan MI
;
Huanhuan LYU
;
Bo XU
;
Gang LI
;
Ji LI
;
Jie HE
;
Zhenhua WANG
- Publication Type:Journal Article
- Keywords:
LiCl;
okadaic acid;
phosphorylation of tau protein;
Alzheimer ’ s disease;
differentiated neurons;
synaptic atrophy;
Giemsa staining
- From:
Chinese Pharmacological Bulletin
2016;32(5):670-675
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the protective effects of lithium chloride ( LiCl ) on neurous injuries and phos-phorylation of tau protein at serine262 induced by okada-ic acid( OA) . Methods The neuroblastoma SK-N-SH cells were differentiated by all-trans-retinoic acid ( AT-RA) . The differentiated SK-N-SH cells were treated with OA to establish the Alzheimer′s disease cellular model. SK-N-SH cells′ viability and proliferation were measured by SRB test. Giemsa staining was used to observe cell morphology. The neurite length of SK-N-SH cells was measured by Image-Proplus software. Syn-aptophysin and phosphorylated tau protein at serine262 expression levels were tested by Western blot. Results The SK-N-SH cells which were treated with 10 μmol ·L-1 ATRA for 7 days displayed mature neuronal fea-tures. The synaptic length of SK-N-SH cells became longer. And the levels of serine262 phospho-tau was sig-nificantly elevated. 20~100 nmol·L-1 OA effectively inhibited the viability of differentiated SK-N-SH cells in a concentration-dependent manner and in a time-de-pendent manner. The OA treatment induced obvious synaptic atrophy in differentiated SK-N-SH cells. And the phosphorylation level of tau protein serine262 also greatly increased. The pretreatment with 10 mmol · L-1 LiCl significantly ameliorated the synaptic atrophy, the decrease of synaptophysin expression and the in-crease of tau phosphorylation at serine262 induced by OA in differentiated SK-N-SH cells. Conclusion LiCl could effectively inhibit OA-induced synaptic atro-phy in differentiated SK-N-SH cells, and it could also result in the increase of synaptophysin expression and the decrease of the phosphorylation of tau protein at serine262 .
