Analysis of CUMS-induced differentially expressed proteins in rat hippocampus by ITRAQ
10.3969/j.issn.1001-1978.2016.05.020
- VernacularTitle:抑郁大鼠海马组织的比较蛋白质组学研究
- Author:
Lisha CAO
;
Fang ZHANG
;
Wen LUO
;
Jing CHEN
;
Miaoquan HE
;
Jisheng WANG
- Publication Type:Journal Article
- Keywords:
ITRAQ;
proteomics;
depression;
hippo-campus;
mass spectrum;
CUMS
- From:
Chinese Pharmacological Bulletin
2016;32(5):697-702
- CountryChina
- Language:Chinese
-
Abstract:
Aims To compare hippocampus between CUMS rats and normal rats, to find differentially ex-pressed proteins and to explore the pathogenesis of de-pression in the protein levels and biological marker. Methods Chronic unpredicted mild stress was taken to establish rat depression model. The ITRAQ-labeled proteins and peptides were separated by the cation col-umn, and differentially expressed proteins were detec-ted and identified by 2D LC-MS/MS. The functions of proteins were analyzed by bioinformatics. Results To-tally 5 109 proteins were identified, 33 differentially expressed proteins were identified, the expressions of 8 proteins were increased and 25 proteins downregulated. Conclusion ITRAQ based sereening is effective in discovering the nosogenesis of depression and new bio-logical marker.
