Determination of ginsenoside Rg1 in intracerebral dialysate by LC-MS/MS and comparison of in vivo and in vitro recovery of microdialysis probe
10.3969/j.issn.1001-1978.2016.05.025
- VernacularTitle:LC-MS/MS法测定脑透析液中人参皂苷Rg1浓度及对其体内和体外探针回收率的考察
- Author:
Yang LIU
;
Wei XUE
;
Min LI
;
Wenyuan QI
;
Yan GAO
;
Xin HU
;
Kexin LI
- Publication Type:Journal Article
- Keywords:
LC-MS/MS;
ginsenoside Rg1;
microdial-ysis;
probe recovery;
in vitro;
in vivo
- From:
Chinese Pharmacological Bulletin
2016;32(5):722-726
- CountryChina
- Language:Chinese
-
Abstract:
Aim To establish a LC-MS/MS method to measure the concentration of ginsenoside Rg1 in intrac-erebral dialysate and compare the probe recovery in vitro and in vivo. Methods The assay was conducted with a ACQUITY UPLC BEH C18(2. 1 mm × 50 mm, 1. 7 μm) . The mobile phase consisted of methanol and ultrapure water and it was detected by gradient elution. The flow rate was 0. 4 mL·min-1 . Specificity, linear range, precision and accuracy, stability were evaluated to investigate the reliability of the method. The recov-ery of ginsenoside Rg1 in probe in vitro and in vivo was compared. Results The retention time of ginsenoside Rg1 was 1. 91 min, the linear range was 0. 1 ~50 μg · L-1 , intra-day and inter-day precisions were less than 15%. The recovery of ginsenoside Rg1 was (4. 05 ± 0. 28)% in vitro and(26. 96 ± 4. 45)% in vi-vo. Conclusion The LC-MS/MS method is accurate, sensitive, and reproducible for quantitative determina-tion of ginsenoside Rg1 in microdialysate. The probe recovery of ginsenoside Rg1 in vivo is higher than in vitro, and both are stable in different concentrations.
