Inhibitory effects of Sanjie Zhentong capsule on primary mouse myometrial cells contraction induced by prostaglandin F2α
10.3969/j.issn.1001-1978.2016.05.027
- VernacularTitle:散结镇痛胶囊对PGF2α诱发小鼠子宫平滑肌细胞收缩的抑制作用探讨
- Author:
Lina LIU
;
Yaozhong LYU
;
Lan SUN
;
Jun ZHOU
;
Zhenzhong WANG
;
Wei XIAO
- Publication Type:Journal Article
- Keywords:
primary mouse smooth muscle cells;
intra-cellular calcium;
contraction;
PGF2α;
CaM;
p-MLC20
- From:
Chinese Pharmacological Bulletin
2016;32(5):732-736
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of Sanjie Zhentong capsule on cultured mouse myometrial cell contraction induced by prostaglandin F2α( PGF2α) , and to elucidate the mechanism of Sanjie Zhentong capsule in treating dysmenorrheal. Methods Primary mouse myometrial cells were cultured and identified. Intracel-lular calcium ( [ Ca2+] i ) was monitored under a Flex-Station 3 Benchtop Multi-Modo Microplate Reader u-sing Calcium 6-QF. Myometrial cells were labeled to observe the changes of contraction. The expressions of calmodulin ( CaM ) , myosin light chain kinase ( ML-CK) , myosin light chain phosphorylation( p-MLC20 ) in mouse uterine smooth muscle cells ( USMCs ) were determined by immunofluorescence. Results Sanjie Zhentong capsule suppressed the intracellular[Ca2 + ]i inflow and reduced the areas of myometrial cells induced by PGF2α. Then it significantly decreased CaM and p-MLC20 levels. Conclusion Our results indicate that Sanjie Zhentong capsule has inhibitory effect on dysmenorrheal induced by PGF2α. Furthermore,its major mechanism may be related with the regulation of intracellular[ Ca2 + ]i inflow and the levels of CaM and p- MLC20.
