Expressions of CD14 mRNA and TLR4 mRNA in peripheral blood mononuclear cells in patients with Par-kinson disease
10.3969/j.issn.1002-0152.2016.02.009
- VernacularTitle:帕金森病患者外周血单核细胞CD14和TLR4中mRNA的表达水平
- Author:
Lingling WANG
;
Si CHEN
;
Jun MA
;
Wei XU
;
Tao YUE
;
Zhenxiang ZHAO
;
Jianfeng WANG
;
Xiaotang WANG
;
Yiming LIU
- Publication Type:Journal Article
- Keywords:
Parkinson disease Monocytes;
RNA;
Messenger TLR4
- From:
Chinese Journal of Nervous and Mental Diseases
2016;42(2):104-108
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression levels of the CD14 mRNA and TLR4 mRNA in peripheral blood mononuclear cells from patients with Parkinson disease and to explore the clinical signicance. Methods For?ty-four patients with Parkinson disease and 37 healthy controls were recruited. We recorded age of onset, duration of ill?ness and sex of all recruited patients. PD patients were evaulated using the Hoehn-Yahr stages, Unified Parkinson Dis?ease Rating Scale (UPDRS) Ⅱand UPDRS Ⅲ,non-motor symptoms scale (NMSS) on“off”time. Reverse transcrip?tion-polymerase chain reaction was performed to determine the expression levels of CD14 mRNA and toll-like receptor 4 (TLR4) mRNA. Results The expression levels of CD14 mRNA(1.459±0.658)2-△△CT and TLR4 mRNA (1.408±0.698)2-△△CT was significantly up-regulated (P<0.05) in Parkinson disease compared with controls((1.162 ± 0.631)2-△△CT、(1.122 ± 0.557)2-△△CT). In addition, there was positive correlation between the expression levels of CD14 mRNA in Parkinson dis?ease patients with the Hoehn-Yahr stages. Meanwhile, there was no significant correlation between the expression levels of CD14 mRNA and TLR4 mRNA with other clinical scores. Conclusions There is positive correlation between the ex?pression levels of CD14 mRNA in Parkinson disease patients with the Hoehn-Yahr stages,indicating that CD14/TLR4 positive monocyte may be involved in the pathogenesis of the Parkinson disease.
