Screening of tumor metastasis related differentially expressed genes in hepatocellular carcinoma cells with FATE/BJ-HCC-2 gene stable transfection by gene chip
10.3969/j.issn.1671-8348.2016.12.004
- VernacularTitle:基因芯片筛选稳定转染FATE／BJ-HCC-2基因的肝癌细胞中肿瘤转移相关差异表达基因
- Author:
Lili GE
;
Xiaoang YANG
;
Junyan PIAO
;
Yanhui YIN
;
Yu ZHANG
- Publication Type:Journal Article
- Keywords:
hepatocelluar neoplasms;
gene chip;
differential gene expression
- From:
Chongqing Medicine
2016;45(12):1595-1597
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen the tumor metastasis related differentially expressed genes in hepatocelluar carcinoma (HCC)cells 7402 after stable transfection with FATE/BJ‐HCC‐2 gene .Methods Total RNA was extracted from FATE/BJ–HCC‐2‐transfected HCC(5B4)cells and empty vector control (Mock)cells respectively .Differentially expressed genes were obtained using cDNA microarray .Results Compared with Mock cells ,a total of 1 694 differentially expressed genes were screened out in 5B4 cells ,the 11 gene expressions had obvious differences ,among which the expression amounts in 7 genes were significantly in‐creased ,including MMP‐1 ,PTGS2 ,FN ,CA9 ,IL‐8 ,ILK and Areg .The fold changes were 81 .80 ,49 .86 ,11 .30 ,16 .26 ,3 .48 ,2 .79 and 2 .20 ,respectively .The expression amounts in 4 genes were significantly decreased ,including E‐cadherin ,E‐cadherin , RHOBTB3 ,ALPP and HLA‐DRB4 .The fold changes were -5 .42 ,-2 .23 ,-5 .93 and -8 .03 ,respectively .Conclusion Adopting gene microarray technology can carefully screen the differentially expressed genes of FATE/BJ‐HCC‐2 involved HCC metastasis ,its final goal is to lay a solid theoretical foundation for studying the HCC metastasis mechanism .
