Influence of siRNA interfering USP7 expression on cytobiological characteristics of laryngeal cancer cells
10.3969/j.issn.1671-8348.2016.12.010
- VernacularTitle:siRNA干扰USP7表达对喉癌细胞生物学特性的影响
- Author:
Ji ZHOU
;
Shishan DENG
;
Dazhong YAN
;
Hai LIU
- Publication Type:Journal Article
- Keywords:
laryngeal neoplasms;
ubiquitin-specific protease 7;
HEP2 cell line;
RNA,small interfering;
apoptosis
- From:
Chongqing Medicine
2016;45(12):1616-1619
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study is to investigate the influences of USP7 on the cytobiological characteristics of laryngeal cancer cells by small interfering RNA (siRNA) interfering the USP7 expression in the laryngeal cancer cells .Methods The self‐de‐signed highly efficient siRNA was used to conduct the specific interference on USP7 expression in laryngeal cancer HEP2 cells . Then the influence on the capacity of cell proliferation and migration ,as well as apoptosise after USP7 interference were observed by using the CCK‐8 method ,Transwell chamber migration test and flow cytometry .Results The self‐designed siRNA could effi‐ciently inhibit the expression of USP7 mRNA in laryngeal cancer cells ,furthermore markedly suppressed the proliferation and mi‐gration of laryngeal cancer cells ,enhanced the cell apoptosis in laryngeal cancer HEP2 cells in vitro .Conclusion The siRNA inter‐fering USP7 can inhibit the proliferation and migration capacity of laryngeal cancer cells ,and promoted their apoptosis .
