Isolation,culture and identification of bi-directional differentiation potential liver stem cells in fetal mice
10.3969/j.issn.1671-8348.2016.12.026
- VernacularTitle:具有双向分化潜能的鼠胎肝干细胞的分离、培养及鉴定
- Author:
Liming YU
;
Shu CHEN
;
Song HE
- Publication Type:Journal Article
- Keywords:
fetal liver stem cells;
centrifugation,density gradient;
cells,cultured;
cell differentiation
- From:
Chongqing Medicine
2016;45(12):1666-1669
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the method of isolating ,culturing and screening fetal mouse liver stem cells in vitro ,and to identify the potential of bi‐directional differentiation .Methods The fetal liver stem cells of mouse were isolated by the density gra‐dient centrifugation and cell difference adherence method ,the proliferation of stem cells was determined by cell plate cloning tech‐nique and MTT method;stem cells were induced for differentiation by adding DMSO and HGF .Results The isolated stem cells showed adherence within 24 h ,which were orbicular‐ovate ,closely packed ,activated within 1~2 weeks ;the positive rates of CD133 , CD49f and EPCAM were (97 .95 ± 1 .21)% ,(92 .71 ± 3 .49)% and (50 .73 ± 3 .45)% respectively ;AFP and CK19 proteins were expressed;red glycogen granules were seen by PAS after induced differentiation;ALB and HNF‐4αwere expressed .Conclusion Fe‐tal hepatic stem cells are successfully isolated by the density gradient centrifugation combined with difference adherence method ,and the isolated cells have strong stemness and proliferation ability ,as well as the ability of bi‐directional differentiation towards hepato‐cytes and bile duct epithelial cells .
