AngⅡ promotes liver fibrosis through PDGF signal pathway

VernacularTitle:血管紧张素Ⅱ靶向调控血小板源性生长因子信号通路促进肝纤维化的形成
Author: Haixia CAO ; Shiyan YAN ; Baocan WANG ; Xiaoyan DUAN ; Jiangao FAN ; Yuqin WANG
Publication Type:Journal Article
Keywords: Ang Ⅱ; HSC; Liver fibrosis; PDGF
From: The Journal of Practical Medicine 2016;32(7):1070-1073
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect and mechanism of AngⅡ on collagen in hepatic stellate cell. Methods HSCs were isolated and cultured, 3H-pro incorporation method was used to evaluate the effects of different doses of AngⅡ on the proline syntheses. RT-PCR assay were used to assess changes in mRNA expression levels of type Ⅰ and Ⅲ procollagen. PDGFR-β mRNA and protein were determined by in situ hybridization and immunocytochemistry. Results 10-8~ 10-5 mol/L AngⅡ could significantly increase the 3H-pro incorporation rate of HSC in a dose-dependent style, 10-6 mol/L AngⅡis the most effective dose. The cultured HSC showed a little expression of type Ⅰ and Ⅲ procollagen mRNAs, while 10-6 mol/L AngⅡwas able to enhance the expression for type Ⅰ and Ⅲ procollagen mRNAs significantly(P < 0.01). AngⅡalso could enhance both mRNA and protein expression of PDGFR-β on HSC(P < 0.01). Conclusion These results suggest that AngⅡ could promote HSC collagen synthesis by enhancing the expressions of PDGFR-β.