Protection effect of dexmedetomidine against H2O2 injury by up-regulating HIF-1α in human renal tubular epithelial cells
10.3969/j.issn.1006-5725.2016.07.016
- VernacularTitle:右美托咪定上调低氧诱导因子-1α对人肾小管上皮细胞H2O2损伤的保护作用
- Author:
Jianbo ZHANG
;
Xiaoqiao WANG
;
Xiaodi QIU
;
Lin RUAN
;
Huansen HUANG
- Publication Type:Journal Article
- Keywords:
Dexmedetomidine;
Hypoxia-inducible factor 1 alpha subunit;
Kidney;
Epithelial cells;
Reperfusion injury
- From:
The Journal of Practical Medicine
2016;32(7):1084-1087
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protection effect of dexmedetomidine against H2O2 injury in Human renal tubular epithelial cells(HK-2 cells). Methods HK-2 cells cultured in vitro were randomly divided into four groups(n = 24): control group, dexmedetomidine pretreatment group, H2O2 injury group, H2O2 injury +dexmedetomidine pretreatment group. Cell viabilities were measured by MTS assay, cell apoptosis were detected using flow cytometry, and expression of HIF-1α protein was quantified by western blot. HK-2 cells were divided into 8 groups by combining with three treatment factors such as PI3K inhibitor LY294002, dexmedetomidine and H2O2 injury. MTS assay was used to detect cell viability and western blot was used to quantify protein expression of HIF-1α,Bcl-2 and Bax after treatment in each group. Results Dexmedetomidine significantly increased the level of HIF-1α、 Bcl-2 in HK-2 cells after H2O2 injury, thus improved viabilities and reduced apotosis of cells. Moreover, effect on H2O2 injury cells of Dexmedetomidine was reversed by PI3K inhibitor LY294002. Conclusion Dexmedetomidine could protect against H2O2 injury by up-regulating HIF-1α expression through activating PI3K/Akt/mTOR signaling pathway in HK-2 cells.
