Study on effect of NSE and U266 cell line on osteoclast-like cells
10.3969/j.issn.1671-8348.2016.10.004
- VernacularTitle:神经元特异性烯醇化酶和U266细胞株对破骨样细胞作用的研究
- Author:
Zhongtao YUAN
;
Chen QING
;
Xiaojin LI
;
Xuemei ZHANG
;
Mingxia SHI
;
Ying HUANG
;
Huimin LI
- Publication Type:Journal Article
- Keywords:
phosphopyruvate hydratase;
myeloma bone disease;
osteoclast;
acid phosphatase
- From:
Chongqing Medicine
2016;45(10):1309-1312
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the functions of neuron‐specific enolase(NSE) and human multiple myeloma U266 cells on osteoclast‐like cells(OLC) function .Methods Normal human peripheral blood mononuclear cells were induced and cultured by adding RANKL and M‐CSF to get OLC ;the experiment was divided into 3 groups ,the NSE group:OLC were cultured in the 6‐well culture plate for 14 d and added with 100 ng/mL recombinant human NSE to culture for 24 ,48 ,72 h;the co‐culture group:OLC were cultured in the lower well of 6‐well Transwell chamber for 14 d ,then added with 1 × 105/well U266 cells in each upper well and conducted the co‐culture for 24 ,48 ,72 h;the control group :OLC were cultured alone .The influences of NSE and U266 cell line on RANKL ,OPG ,IL‐6 and TRAP mRNA transcriptional level of OLS were compared by using real‐time fluorescent quantitative PCR .Results RANKL ,OPG ,IL‐6 mRNA had no expression on OLC in the co‐culture group ,NSE group and control group ;com‐pared with control group ,the TRAP mRNA expression level in the co‐culture group and the NSE group was increased ,the differ‐ence was statistically significant(P<0 .01);the increase of TRAP mRNA expression level was obvious especially at 48 ,72 h .Con‐clusion OLC expressing TRAP and NSE may be one of the factors for promoting OLC differentiation and maturation in myeloma bone disease ,prompting that NSE could increase the OLC viability .
