Scanning the SRSF2 gene mutations in acute myeloid leukemia by using high-resolution melting analysis
10.3969/j.issn.1006-5725.2016.07.042
- VernacularTitle:高分辨率熔解分析检测急性髓系白血病SRSF2基因突变
- Author:
Dongming YAO
;
Jing YANG
;
Zhen QIAN
;
Lei YANG
;
Gaofei XIAO
;
Jiang LIN
;
Hong GUO
;
Jun. QIAN
- Publication Type:Journal Article
- Keywords:
AML;
High-resolution melting analysis;
SRSF2 mutation;
Prognosis
- From:
The Journal of Practical Medicine
2016;32(7):1169-1172
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the SRSF2 mutations in acute myeloid leukemia (AML) patients by using high-resolution melting analysis (HRMA). Methods PCR-HRMA analysis was performed to screen SRSF2 mutations in 140 cases with AML, and the direct DNA sequencing was used to confirm the HRMA results. Results Five percent (7/140) of AML patients were found with heterozygous SRSF2 mutations, including one case of P95R mutation, two case of P95L mutation, and four cases of P95H mutation, the above mutations were confirmed by direct DNA sequencing. The maximal sensitivity of HRMA in detecting SRSF2 mutation was close to 10%. There were no difference in gender, age and blood parameters among cases with or without SRSF2 mutations (P > 0.05). The overall survival (OS) of patients with SRSF2 mutations was inferior to those without SRSF2 mutations in AML patients (P=0.016). Conclusions HRMA analysis was a convenient, rapid, specific, high-throughput technique for scanning of SRSF2 gene mutations in AML patients. SRSF2 mutation may predict the adverse prognosis in AML patients.
