Study on colonic H2 S-producing enzyme CSE and colonic motility in diabetes mellitus
10.3969/j.issn.1671-8348.2016.11.007
- VernacularTitle:肠道硫化氢合成酶CSE与糖尿病大鼠结肠动力的相关研究
- Author:
Qin WANG
;
Ying LIU
;
Zhong LIN
;
Zehui DUAN
- Publication Type:Journal Article
- Keywords:
hydrogen sulfide;
diabetes mellitus;
cystathionine-γ-lyase;
colonic motility
- From:
Chongqing Medicine
2016;45(11):1463-1466
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationsheep between colonic H2 S‐producing enzyme cystathionine‐γ‐lyase(CSE) and colonic motility in a rat model of diabetes mellitus(DM) .Methods To obtained diabetic rat models ,all rats were injected intra‐peritoneally (ip) 1% streptozotocin (STZ ,65 mg/kg) ,type 1 diabetes model was established ,nornmol conditions were observed pe‐riodically .Ten days after treatment ,production of colonic longitudinal smooth muscle strips ,organ bath recordings were used to test the colonic motility ;immunofluorescence and double immunofluorescence lableling method were performed to detect the distribution of CSE;Western blot were performed on rat colonic samples devoid of mucosa and submucosa to detect the expression of CSE .Re‐sults DM rats decreased the colonic motility(P<0 .05);CSE was strongly expressed in the cytosols of the circular and longitudinal smooth muscle cells and the nucleus of the myenteric plexus neurons ;CSE distributed in myenteric neurons in the nucleus of rat ,but the proportion of MP had no difference (P>0 .05);DM rats increased the expression of CSE in the colon devoid of mucosa and sub‐mucosa(P<0 .05) .Conclusion The decrease of colonic motility in diabetic rat may be associated with the increased production of H2 S‐producing enzyme CSE .
