Expression of GABA and glutamate vesicular transporter in depressed mice
10.3969/j.issn.1671-8348.2016.11.009
- VernacularTitle:抑郁小鼠大脑GABA 及谷氨酸囊泡转运蛋白表达变化
- Author:
Qiong WU
;
Jingfang SONG
;
Xiaoling ZHANG
;
Hongyang CAI
;
Quanzhong CHANG
- Publication Type:Journal Article
- Keywords:
depression;
GABA transporter;
vesicular glutamate transporter
- From:
Chongqing Medicine
2016;45(11):1470-1472
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of vesicular GABA transporter and vesicular glutamate transporter 1 in de‐pression .Methods Mice was divided into control group and defeat group stochastically .By social defeat model and social avoidance , the defeat group was divided into two groups:susceptible group and unsusceptible group .Synaptic proteins were extracted respec‐tively from the 3 groups .We detected the expression abundance of VGAT and VGLUT1 by Western blot .Results Compared with the control group ,in susceptible group ,the residence time in the contact area was significantly reduced ,and the residence time in the corner area was significantly increased ,with statistical difference(P<0 .05) .In the prefrontal cortex and hippocampus ,Com‐pared with the control group and the unsusceptible group ,the expression levels of VGLUT1 and VGAT were increased in the sus‐ceptible group(P<0 .05) .there were no statistically significant in VGLUT1 and VGAT leveles between control group and the un‐susceptible group(P>0 .05) .In the striatum ,although the expression levels of VGAT and VGLUT1 were increased in susceptible group ,but in unsusceptible group ,the expression of these proteins also increased significantly .Conclusion The prefrontal cortex and hippo‐campus excitability and inhibitory vesicle transport were changed in depression ,which may relate to the transcription disorder .
