Effect of Vagus Nerve Stimulation on Expression of 5-hydroxytryptamine 2A Receptor in Prefrontal Cortex of Coma Rats with Traumatic Brain Injury
10.3969/j.issn.1006-9771.2016.04.007
- VernacularTitle:迷走神经电刺激对脑外伤昏迷大鼠前额叶皮质5-羟色胺2A受体表达的影响
- Author:
Xiaoyang DONG
;
Zhen FENG
- Publication Type:Journal Article
- Keywords:
traumatic brain injury;
coma;
vagus nerve stumulation;
5-hydroxytryptamine 2A receptor;
orexin-A;
rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2016;22(4):404-408
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of vagus nerve stimualtion on wake-promoting and the expression of 5-hydroxytryptamine (5-HT) 2A receptor in the prefrontal contex of coma rats with traumatic brain injury. Methods 72 Sprague-Dawley rats were randomly divid-ed into control group, sham-stimulated group, stimulated group and antagonist group with 18 rats in each group. Traumatic brain injury mod-el was established by a weight-drop head injury. The antagonist group was injected with SB334867, and both the antagonist group and the stimulated group received vagus nerve stimulation. Their behaviors were recorded. And immunohistochemistry technique was used to detect the expression of 5-HT2A receptor in the prefrontal cortex. Results 12 rats in the stimulated group, 9 in the antagonist group and 4 in the sham-stimulated woke up. The expression of 5-HT2A receptor from low to high was ranged as the control group, the antagonist group, the sham-stimulated group and the stimulated group (χ2=11.464, P=0.009). Conclusion Vagus nerve stimulation could raise consciousness in co-ma rats after traumatic brain injury, which may be related to up-regulating the expression of 5-HT2A receptor.
