Application of Two Kinds of Tissue Clearing Method in Observing 3D Imaging of Spinal Cord with Immunofluo-rescent Staining

VernacularTitle:两种组织透明技术在免疫荧光染色观察脊髓3D结构中的应用
Author: Hongmei DUAN ; Junkui SHANG ; Peng HAO ; Qianqian LI ; Feixiang YANG ; Zhaoyang YANG ; Xiaoguang LI
Publication Type:Journal Article
Keywords: spinal cord; transparency; axon regeneration; CUBIC; iDISCO; rats
From: Chinese Journal of Rehabilitation Theory and Practice 2016;22(4):417-421
CountryChina
Language:Chinese
Abstract: Objective To compare the application of CUBIC and iDISCO clearing methods in observing 3D imaging of spinal cord with immunofluorescent staining. Methods 1 mm thick spinal cord coronal sections were processed with CUBIC and iDISCO, respectively. The neurofilament (NF) protein was detected by immunofluorescent staining and then was observed by a laser confocal microscope. Results Compared with CUBIC, iDISCO had the advantages of shorter time, higher transparency (F=6.64, P<0.01), and deeper penetration (F=5117.55, P<0.01). Conclusion Immunofluorescent staining combined with iDISCO could completely observe the spinal axons with shorter time and better stain effect.