Donepezil promotes neurogenesis in the subventricular zone：an experimental study of cerebral ischemia in mice
10.3969/j.issn.1006-5725.2016.07.004
- VernacularTitle:多奈哌齐促进脑梗死后室管膜下区神经发生的实验研究
- Author:
Jianping WANG
;
Di ZHANG
;
Dongdong JIN
;
Xiaobing CUI
;
Zhengfang LU
;
Haibo GUAN
- Publication Type:Journal Article
- Keywords:
Brain ischemia;
Donepezil;
Subventricular zone;
Neurogenesis
- From:
The Journal of Practical Medicine
2016;32(7):1048-1050
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of donepezil on neurogenesis in the subventricular zone (SVZ) under the cerebral ischemic conditions. Methods Sixty mice were randomly assigned to a sham +vehicle group, a middle cerebral artery occlusion(MCAO) + vehicle group and a MCAO + donepezil group, 20 in each group. A model of MCAO was established. Neural stem cells/progenitor cells were labeled by Mash1, and neuroblasts were labeled by doublecortin (DCX). The expressions of DCX in the SVZ cells of each group were detected by immunofluorescence. The expressions of Mash1 and DCX in the SVZ cells of each group were quantified by Western blot. Results 10 days after MCAO was undergone, DCX-positive cells were seen in the SVZ of each group, and the expressions of DCX and Mash1 in MCAO + vehicle group were markedly increased as compared with the sham + vehicle group (P < 0.05), and that in MCAO + donepezil group were significantly increased as compared with the other two groups (P < 0.05). Conclusion As a kind of acetylcholinesterase inhibitors, donepezil can promote neurogenesis in SVZ under the cerebral ischemic conditions.
