Transformation and standardization of the inactivation test method for virus suspension in biosafety laboratory
10.3969/j.issn.1671-7856.2016.02.006
- VernacularTitle:生物安全实验室病毒悬液灭活试验方法标准转化研究
- Author:
Amei DENG
;
Feng WEI
;
Jia WU
;
Haixia MA
;
Zhiming YUAN
- Publication Type:Journal Article
- Keywords:
Virus inactivation;
Chemical disinfectant;
Biosafety;
Standard;
Quantitative suspension test;
Virucidal effect evaluation
- From:
Chinese Journal of Comparative Medicine
2016;26(2):27-32
- CountryChina
- Language:Chinese
-
Abstract:
Chemical disinfectants are generally used for virus inactivation and environment disinfection in biosafety laboratory, and the efficacy and evaluation of the disinfection are critical to ensure the laboratory biosafety.However, there is a current lack of applied standard to evaluate the virucidal efficacy of chemical disinfectants in our country.In this paper, a European Union standard“Method and Requirements of Virucidal Quantitative Suspension Test Method for Chemical Disinfectants Used in Human Medicine” was analyzed and a standard transformation scheme has been proposed.It is suggested that the model viruses should be increased from 3 to 6, including the surrogate viruses to substitute highly pathogenic viruses, and that the method to remove the residual chemical disinfectant and the calculation of 95%confidence interval should be incorporated into the standard.The suggestion will improve the scientific and operational standards related to disinfection and sterilization in biosafety laboratory in China.
