Expression of Aquaporin-4 in White Matter of Spinal Cord in Rats after Spinal Cord Contusion

VernacularTitle:脊髓损伤后水通道蛋白-4在脊髓白质中的表达
Author: Hongyu XIANG ; Shengnan LI ; Xi ZENG ; Xiao ZHANG ; Nan LIANG
Publication Type:Journal Article
Keywords: spinal cord contusion; edema; aquaporin-4; rats
From: Chinese Journal of Rehabilitation Theory and Practice 2016;22(4):428-432
CountryChina
Language:Chinese
Abstract: Objective To explore the expression of aquaporin (AQP)-4 in white matter of spinal cord after spinal cord contusion (SCC). Methods 88 adult Sprague-Dawley rats were assigned randomly to sham operation group and SCC group. The model was established by Al-len's method. BBB sore was used to assess the motor function of rats. The relative expression of AQP-4 mRNA was determined by Q-PCR technique. The localization of AQP-4 was observed by immunohistochemistry. Results BBB score showed motor dysfunction in SCC group, and it increased 7 and 14 days after SCC (t>5.061, P<0.001). The level of AQP-4 mRNA decreased on the 1st and 3rd days (t>50.44, P<0.001), and increased on the 5th day (t=-3.968, P=0.001), and lasted until the 28th day (t=-4.227, P=0.001) compared with that on the 3rd day. The immunohistochemistry showed AQP-4 was located on the process of glial cell and vascular endothelial cells in white matter of spi-nal cord. Conclusion AQP-4 may play various roles at different stages in SCC.