Metabolic Profile of Dyskinetic Cerebral Palsy Based on Metabonomics
10.3969/j.issn.1006-9771.2016.04.016
- VernacularTitle:不随意运动型脑性瘫痪血清代谢轮廓的特征分析
- Author:
Junchen CHEN
;
Xia HE
;
Yu DUAN
;
Yunchuan PENG
;
Mingwei ZHANG
- Publication Type:Journal Article
- Keywords:
cerebral palsy;
dyskinetia;
metabolomics;
nuclear magnetic resonance spectroscopy;
partial least square discriminant analy-sis
- From:
Chinese Journal of Rehabilitation Theory and Practice
2016;22(4):448-454
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the metabolic profile of children with dyskinetic cerebral palsy by metabolomics, and its abnormal metabolic pathway. Methods The serum of 10 children with dyskinetic cerebral palsy (patient group) and 7 healthy children (control group) aged 6 to 12 years were collected at clinic from May to August, 2014. The serum samples were tested by the nuclear magnetic resonance spectrometer and the spectroscopies were discriminated by partial least squares-discriminant analysis. According to the human metabolome database, the final metabolites disturbed would be figured out. Results 15 chemical shifts were defined, and 6 of them, including 2.04 ppm, 2.12 ppm, 3.00 ppm, 3.24 ppm, 3.76 ppm, 6.50 ppm, were significantly different between 2 groups (P<0.05). The KEGG Pathway Database showed that the levels of taurine, fumarate, oxaloacete, pyruvate, citrate, aspartate, succinate, malate, cysteine decreased, and the levels of glutamate, 2-oxoglutarate, glutamine, leucine, alanine increased. The abnormal metabolism was found in taurine metabolism, glutamine me-tabolism and energy metabolism pathways. Conclusion Based on metabolomics, the metabolic profile of children with dyskinetic cerebral palsy was discriminated out successfully. The further research can focus on the small molecules found out.
