Overexpression of β3-adrenoreceptor aggravates cardiac hypertrophy
10.3969/j.issn.1006-5725.2016.08.006
- VernacularTitle:β3－肾上腺素能受体基因过表达促进心肌细胞肥大
- Author:
Hui ZHANG
;
Li WANG
;
Yuan ZHANG
;
Yitong MA
;
Yining YANG
;
Miaomiao MA
- Publication Type:Journal Article
- Keywords:
Receptors,adrenergic,beta-3;
Cardiomyocyte;
Lentivirus;
Norepinephrine;
Cardiac hyper-trophy
- From:
The Journal of Practical Medicine
2016;32(8):1219-1222
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of β3-adrenoreceptor (β3-AR)overexpression on cardiac hypertrophy. Method Sprague-Dawley rat neonatal ventricular cardiomyocytes (NRVMs) were isolated and cul-tured in vitro.The infection of lentiviruswas examined after cardiomyocytes were infected with lentivirus at differ-ent multiplicity of infection (MOI) of 20、50、80 and 100. Fluorescence microscopy was used to observe the ex-pression of GFP and to confirm the best MOI for lentivirus infection. Following the enforced expression of β3-AR by lentivirus, 2uM norepinephrine (NE) was used to treatment the infected cardiomyocytes for 48h. Expressions of β3-AR、c-myc and c-fos protein in cardiomyocytes were detected by Western Blot. Results The results of fluorescence microscopy indicated that the best MOI was 50. The protein level of β3-AR was significantly in-creased in β3-AR overexpression group compared with the control group and the NE treatment group (P < 0.05). Following the treatment of NE , the expressions of c-myc and c-fos were also significantly increased in β3-AR overexpression group compared with the control group (P < 0.05). Conclusion Over-expression of β3-AR can aggravate cardiomyocyte hypertrophy.
