Effect of lipopolysaccharide on expression of Robo4 in human umbilical vein endothelial cells
10.3969/j.issn.1006-5725.2016.08.009
- VernacularTitle:脂多糖对人脐静脉内皮细胞Robo4表达的影响
- Author:
Lin WANG
;
Rongxiu ZHENG
- Publication Type:Journal Article
- Keywords:
Lipopolysaccharides;
Human Umbilical Vein Endothelial Cells;
Roundabout 4 (Robo4)
- From:
The Journal of Practical Medicine
2016;32(8):1230-1232
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Lipopolysaccharide (LPS) in different concentrations on expression of Robo4 in human umbilical vein endothelial cells (HUVECs). Methods HUVECs were cultured in vitro and randomly divided into 3 groups: control group, low dose LPS group (10 μg/mL) and high dose LPS group (100 μg/mL). Robo4 protein level was detected by immunofluorescence staining and western blot , and Robo4 mRNA level was measured by real-time PCR. Results Robo4 protein and Robo4 mRNA in high dose LPS group were 0.49 ± 0.08 and 0.23 ± 0.08 respectively , which were significantly decreased than those (1.35 ± 0.15 and 0.97 ± 0.17) in control group(P < 0.05). However, there were no significant difference between the low dose (1.23 ± 0.13 and 0.94 ± 0.14) group and the control group (P > 0.05). Conclusion High dose LPS (100 μg/mL) could down-regulate expression of Robo4 in HUVECs.
