Protective Effect of Reduced Glutathione on Kidney against Acute Ischemia-reperfusion Injury
10.3969/j.issn.1006-5725.2016.08.010
- VernacularTitle:还原型谷胱甘肽对肾急性缺血再灌注性损伤的作用
- Author:
Yan LIN
;
Tao HE
;
Xiaoyan MAO
;
Zhonglin GAN
;
Li ZHANG
;
Jing HOU
- Publication Type:Journal Article
- Keywords:
Reduced glutathione(GSH);
Pretreatment;
Kidney;
Ischemia-reperfusion injury
- From:
The Journal of Practical Medicine
2016;32(8):1233-1236
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the pretreatment and post-treatment effect of reduced glutathione (GSH) on acute ischemia-reperfusion injury (IRI) of rat kidney. Methods Fifty adult SD rats were divided into 5 groups: control group (Sham group), ischemia reperfusion group (I/R group), GSH pretreatment group (pre-treatment group), and GSH post-processing group (post-treatment group), with ten rates in each group. Animals in pre-treatment group were injected 4% GSH 100 mg/kg intraperitoneally at 24th , 16th , 8th hour and 45th minute before surgery. Animals in post-treatment group were administrated GSH with the same dosage at 45th minute, 6th, 12th and 18th hour after surgery. Creatinine (Cr), urea nitrogen (BUN) level, the total superox-ide dismutase (T-SOD) activity, malondialdehyde (MDA) and nitric oxide (NO) levels in serum were measured at 24th hour after surgery. Histopathological changes were checked by H. E staining. Results Damage on kid-ney structure of animals in pre-treatment group was less than that in I/R group. There was little pathological change on kidney of those in pre-treatment group. Serum Cr, BUN, MDA and NO levels were all decreased but T-SOD activity increased in pre-treatment and post-treatment group when compared with those in I/R group (P <0.05), (P < 0.05). T - SOD activity in post-treatment group was higher than that in pre-treatment group (P <0.05). Conclusion GSH can protect rats against acute renal ischemia-reperfusion injury within 24 hours before and after kidney ischemia-reperfusion , especially after ischemia-reperfusion.
