Role of cancer stem cells in EMT-induced acquired resistance to EGFR-TKIs in NSCLC cells
10.3969/j.issn.1006-5725.2016.08.007
- VernacularTitle:肿瘤干细胞在NSCLC细胞EMT介导的EGFR-TKI获得性耐药中的作用
- Author:
Qiaoting HU
;
Juan ZHOU
;
Donghai CHENG
;
Weimin ZHANG
- Publication Type:Journal Article
- Keywords:
Non-small cell lung cancer;
Epithelial-mesenchymal transition;
Epidermal growth factor receptor-tyrosine kinase inhibitors;
Acquired resistance;
Cancer stem cell
- From:
The Journal of Practical Medicine
2016;32(8):1223-1225
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of cancer stem cells in EMT-induced acquired resistance to EGFR-TKIs in NSCLC. Methods The EGFR del E746-A750 mutated human lung adenocarcinoma PC-9 cell line and gefitinib acquired resistance cell line PC-9/AB were used in this study. EMT was assessed by western blotting assay. The sensitivity to gefitinib was tested with CCK8. Flow cytometry for antibody analysis was used to quantify CSCs within the cell lines. Results Compared with PC-9, PC-9/AB underwent EMT and showed no-table resistance to gefitinib (P < 0.01). Compared with PC-9, the proportions of CSCs were much higher in PC-9/AB. Conclusion EMT plays an important role in the acquired resistance to EGFR-TKIs in NSCLC , possibly through the up-regulation of CSCs.
